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Sample extracts were placed briefly on a TurboVap (Zymark, Hopkinton, MA, USA) to remove the organic solvent and subsequently stored overnight under nitrogen before preparation for analysis

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Carnitine deficiency is defined biochemically as abnormally low plasma concentrations of free carnitine, less than 20 mol/L at one week post term and may be associated with low tissue and/or urine concentrations

However, choosing a senior food formulated for adult maintenance or all life stages ensures the recipe fulfills the recommended protein, fat, and nutrient requirements of adult dogs