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Following the addition of an ABC reagent and a subsequent substrate solution, the resulting colorimetric change was quantified by measuring the absorbance at a specific wavelength

Methods Peptide agonists and general reagents Rat amylin (rAmy), salmon calcitonin (sCT) and human calcitonin (hCT) were purchased from China Peptides

The most abundant non-fluorescence AGEs involve carboxymethyl-lysine (CML), and carboxyethyl-lysine (CEL)