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The peptide activates extracellular signal-regulated kinase 1/2 (ERK1/2) phosphorylation in a dose-dependent manner, leading to nuclear translocation and activation of transcription factors including c-Fos, c-Jun, and early growth response-1 (Egr-1) that drive expression of genes involved in cell growth, migration, and angiogenesis

However, this type of study measures correlation

A May 2025 study in Molecular Therapy from the INc-UAB showed that gene therapy increasing secreted klotho (s-KL) in mice extended lifespan by 15-20% while improving muscle, bone, and cognitive health at 24 months of age (equivalent to ~70 human years)