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Similar to eIF2 phosphorylation, ATF4 protein expression peaked at 7 h and drastically decreased, although still significantly increased compared to control cells, after 24 h of 4CBA treatment (Fig

These results indicate that the cell structure and function were normal

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Constipation was assessed by frequency of defecation, response to laxatives and feces forms which were classified according to Bristol stool scale, (type 1 = separate hard lumps) (type 2 = sausage shaped and lumpy) (type3 = sausage shaped with cracks on its surface) (type 4 = sausage or snake with smooth surface) (type 5 = soft blobs) (type 6 = fluffy pieces) (type 7 = entirely liquid) 17