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Alcoholic liver disease: Chronic alcohol consumption severely depletes hepatic glutathione through multiple mechanisms: increased ROS production during ethanol metabolism, reduced cysteine availability, and impaired mitochondrial glutathione transport

Methods: A literature search was performed using MEDLINE/PubMed, focusing on publications of antioxidant therapies for iOAT

At day 3 of adulthood worms were picked, washed with PBS and stained with 5 M of dihydroethdium (DHE, Invitrogen) in PBS for 45 min at 20 C with agitation

325,326 This improvement is achieved through the inhibition of ferroptosis by downregulating ACSL4 expression via a PPAR--independent pathway