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Description
The column was washed with lysis buffer (5 mM imodazole in lysis buffer) and increasing concentrations of NaCl (0.5 mM and 1 mM) followed by increasing concentrations of imidazole (5 mM and 20 mM, in lysis buffer) to remove contaminating proteins

However, if split dosing improves your adherence and reduces side effects, the practical benefit may outweigh theoretical pharmacokinetic concerns

Never shake vigorously, as this can damage the peptide structure

[DOI] [PMC free article] [PubMed] [Google Scholar] 39.Liu, L
