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Cells were harvested and lysed in lysis buffer (50 mM Tris-HCl, pH 7.5, 300 mM NaCl, 10 mM imidazole, and 2 mM PMSF) using a Pulse 150 Ultrasonic Homogenizer with 6 probe (Benchmark Scientific, Sayreville, NJ)

Definitions of hypertension, hyperuricemia, dyslipidemia and diabetes According to the Japanese guideline for hypertension 21 , individuals with at least one of the following findings are diagnosed as having hypertension: (1) office blood pressure levels of 140/90 mmHg or more and/or (2) out-of-office blood pressure levels of 135/85 mmHg or more

In pregnant women, IF seems safe to be conducted and possibly useful to treat endocrine disorders during pregnancy

Joint Pain and Muscle Aches: Bromelain's proteolytic action breaks down inflammatory fibrin deposits in tissues, while NAC reduces the oxidative stress that contributes to widespread, chronic musculoskeletal pain