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After 5 min only one peak corresponding to GSSG was observed

This was because these cells efficiently reduced free Met S -SO by MsrA and utilized the resulting Met, whereas free Met- R -SO was not reduced by MsrBs( 48)
Typically, serum Se concentrations of 90100 g/L are required to achieve optimal GPX activity, although values closer to 125 g/L may be necessary to achieve complete saturation of SelP
Oncotarget 7 , 1175611769 (2016)
