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An additional 700 l of 1X Lysis/Binding/Wash Buffer with a total of 1.5 mg protein was added to the beads in each tube and incubated overnight at 4 C with gentle rotation
10.1016/j.redox.2014.12.011 177 ZilkaO.ShahR.LiB.Friedmann AngeliJ
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Briefly, HT-1080 cells were seeded into 15-cm dishes at a density of 1 10 6 cells/dish, then the next day treated nutlin-3 (10 M)
