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These effects may be mediated through glial and anti-inflammatory mechanisms, contributing to the neuroprotective properties of rapamycin (Figure A)

Bacteriostatic water (which contains a small amount of benzyl alcohol as a preservative) is generally preferred over sterile water for multi-use vials because it inhibits microbial growth over the days a reconstituted vial is in use

Laurenza I, Colognato R, Migliore L, et al

Implications of cryopreservation on structural and functional attributes of bovine spermatozoa: an overview
