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Heli H, Mirtorabi S, Karimian K

The diminished function of key antioxidant enzymes and the overproduction of ROS/RNS can disrupt insulin signaling pathways, exacerbating hepatic lipid accumulation

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Based on prior experience with this kit, to ensure proper mixing of reagents, we placed the 96-well plate on an orbital plate shaker during incubation steps