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The blue dotted lines are called the confidence interval, the closer they are to the actual plotted line (green line) the surer we are of the data, meaning the more data points we had in this region to ensure accuracy

Poly(acrylic acid)-cysteine-2-mercaptonicotinic acid (PAAcys-2MNA) conjugates were synthesized by the oxidative disulfide coupling of PAA-cys (100-, 250- and 450 kDa) with 2-mercaptonicotinic acid (2MNA)

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