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Researchers prevent this by immediately deproteinizing samples with acidic solutions such as metaphosphoric acid (MPA) or perchloric acid (PCA) and processing all materials on ice

Midrange complaints included distension (69.8%), hard stool (67.9%), and decreased passage of stool (65.4%)
American Journal of Preventive Medicine 2015;48(6):e1126

Developmental trajectory of the healthy human gut microbiota during the first 5 years of life
