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These quantitative tests can also identify heterozygous females better

Over time, the constant toxic load from the yeast byproducts can overwhelm the liver and other detoxification organs, leading to chronic fatigue, brain fog, and mood disorders like anxiety and depression
doi: 10.1128/IAI.00497-10 318 TakahashiH.WatanabeH.KimK

The plasmid containing the desired mutations, confirmed by sequencing results, was then transformed into a BL21-CodonPlus (DE3) RIL strain
