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The concentration of IFN and CCL5 in collected culture supernatants was measured using LEGEND MAX Mouse IFN- ELISA Kit (#439407, BioLegend) and Mouse RANTES ELISA Kit (#E-EL-M0009, Elabscience Biotechnology Co., Ltd) according to the manufacturer's instructions

3.2.2 The Met/cystine cycle and other amino acid pathways Beyond the direct uptake of extracellular cystine, cells can also generate cysteine via the methionine (Met) cycle and transsulfuration pathway (86)

Yan R, Xie E, Li Y, Li J, Zhang Y, Chi X, et al

Esculentoside A suppresses Abeta(142)-induced neuroinflammation by down-regulating MAPKs pathways in vivo