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German acupuncture trials (GERAC) for chronic low back pain: randomized, multicenter, blinded, parallel-group trial with 3 groups
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2) Resuspend the cells in 1/10 volume of Lysis Buffer with appropriate amount of protease inhibitors added, which must have no effect on the binding capacity of agarose
This involves Ca 2+ /calmodulin-dependent activation of neuronal nitric oxide synthase (nNOS) and conversion of L-arginine to NO
