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zonata ZON001 Classic specimen EIF3L PCR-RFLP Test 1 BsrI: 600, 200 HinfI: Does not cut HhaI: 680, 190 Sau3AI: Does not cut SnaBI: 535, 330 SspI: 750, 120 Vspl: Does not cut Approximate ITS1 fragment length gel: 850 bp PCR-RFLP Test 2 No fully diagnostic restriction enzymes, but a combination of several can be chosen to distinguish B

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Indeed, the mutant lacking both PLCs was severely impaired in its ability to escape from the primary vacuoles and cellcell spread and was 500-fold less virulent ( Levels of DAG and ceramide were substantially increased in the host cells 4 to 5 h postinfection with L