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Cells were centrifuged at 2300rpm for 25 minutes at 20C, with brakes off
Shen Z, Song J, Yung BC, Zhou Z, Wu A, Chen X
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The formation of fibrillar aggregates was characterized by the half-time of aggregation (T), defined as the time required to reach 50% of the maximum Th-T fluorescence intensity, as derived from fitting the kinetic curves to a one-phase exponential association model
