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Fluorescence recovery after photobleaching Run-off in vitro transcription (IVT) of GLS intron 14 or empty vector were performed using Riboprobe in vitro transcription system (Promega), from sequences cloned into pGEM-T easy/BamHI, expressed from T7 promoter

To address these challenges, advanced nanotechnology-based drug delivery systems such as nanoparticles, liposomes, micelles, and polymeric carriers have been developed to enhance its solubility, stability, and targeted bioavailability