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Description
To generate a longer repeat size, the pMD20-( G 4 C 2 ) 50 vector was digested with Eag I and PspOM I, followed by ligation into the pMD20-( G 4 C 2 ) 50 vector linearized by digestion with Eag I

The lysine moiety of the fragment was positioned near Asp271, Ser336, Phe337, Asp340, Glu368, Asp370, and Glu384

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You want to maintain steady levels in your system rather than having huge spikes and dips
