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1:100), anti-GRASP65 antibody as a Golgi marker (Santa Cruz Biotechnology, sc-374423

The 2D culture models of skeletal muscle cells have several advantages such as the simplicity and low-cost of maintaining cells in culture, the short time required to obtain the fusion of myoblasts into multinucleated myotubes (2 to 3 days) and the easy use of genetic manipulation [57, 97]

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