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In this article: Nickel affinity chromatography Nickel bead binding capacity and buffer compatibility What the color of the nickel beads tells me about my purification Comparing with other affinity chromatography techniques Nickel is conjugated to agarose beads using one of two chelating agents: iminodiacetic acid (IDA) or nitrilotriacetic acid (NTA) (Figure 1)

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It occurs endogenously in humans, where it has been identified in the hypothalamus, the limbic system, the pituitary, and even the gastrointestinal tract