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Functional manipulations included targeted knockdown and overexpression of Cck in mPFC excitatory neurons via viral-mediated gene transfer

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Research shows activation of genes involved in: Collagen I & III synthesis Integrins, ECM remodeling enzymes Nerve regeneration and axonal repair Antioxidant defense Anti-cancer and anti-senescence pathways This makes GHK-Cu a valuable model compound for studying epigenetic regulation of healing processes

Filtrates were centrifuged in an Eppendorf 5810R at 1,200 RPM (290 x G) for 8 min, supernatant was aspirated, and the remaining pellet was resuspended in BMDM media