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The following qRT-PCR conditions were used: 40 cycles of 94 C for 15 s, annealing temperatures as indicated in Table (1) for 30 s, and extension temperature at 72 C for 20 s were performed after 95 C for 4 min

Sarzi-Puttini P, Giorgi V, Atzeni F, Roberto G, Kosek E, Choy E, Bazzichi L, Hauser W, Ablin J, Aloush V, Buskila D, Amital H, Da Silva J, Perrot S, Morlion B, Polati E, Schweiger V, Coaccioli S, Varrassi G, Batticciotto A

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While these indicator tests do not directly detect disease-causing pathogens, they provide an early warning of potential contamination and help verify strong manufacturing and sanitation controls