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To examine BAT CoQ deficiency in vivo, we used a brown/beige fat-specific knockout of the CoQ synthesis enzyme decaprenyl diphosphate synthase subunit 2 (PDSS2) by crossing UCP1-cre animals with a PDSS2 Flox (Peng et al, 2008) strain, both in the C57 background, generating the PDSS2 BKO line
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