what role does glutathione play in detoxification processes The Science of Detoxification: How Phase I Relates to Health Glutathione Metabolism - an overview
Description
His-tags have lower binding specificity compared to strep-, FLAG-, and GST-tags, which is the major limitation of nickel affinity chromatography (Figure 5) (Kimple et al, 2015)
Ideal candidates is anyone with body mass index (BMI) above 30 or with a BMI over 27 and at least one weight-related comorbidity, such as type 2 diabetes, hypertension, or sleep apnea
doi: 10.1073/pnas.82.23.8232 111 ProchaskaH
Elution conditions for other affinity tags are also generally well tolerated by proteins, so this really isnt a reason to try one tag or another first
