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If youre sensitive to smells or you get headaches from fragrance, this is gonna annoy the hell out of you

After fixation with 1% osmium acid solution at room temperature for 1.5 h, the samples were washed three times with 0.1 M phosphate buffer and gradually dehydrated in ethanol (50-100%)

Then, careful layering of 2.6 mL each of 1.15 M, 0.86 M, and 0.25 M sucrose solutions was performed on the microsomal suspension, and this step gradient sucrose solution was centrifugated at 82,000 g for 3 h at 4 C

449,450,451 For example, Li et al
