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For domain titration, 100 M isotopically labelled a 0 or b in the presence of 20 mM CaCl 2 was mixed with 200 M unlabelled b or a 0 in the same buffer solution

Aside from that, Enzymatic activity causes localized dark patches

Enhanced proliferation and osteogenic differentiation of mesenchymal stem cells on graphene oxide-incorporated electrospun poly(lactic-co-glycolic acid) nanofibrous mats

Similarly, N-terminal truncation in BpGGT up to 95 residues did not affect autoprocessing, but functional activity was lost with truncation beyond 63 residues (Murty et al., 2012)
