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Chondrocyte dedifferentiation was majorly characterized as the reduced expression of COL2 and ACAN and enhanced expression of type I collagen and versican ( COL2 expression, but not ACAN ( In addition to dedifferentiation, another consequence of in vitro expansion is replicative senescence, which also significantly influences chondrocyte function

Dulbecco's modified Eagle's medium for cell culture, fetal bovine serum, and trypsin were purchased from Gibco (Grand Island, USA)

The 10mg vial is ideal for the Phase 1 starting dose

Disadvantages If the injection misses the muscle and is not deposited into fat then some or all of the medicine might be wasted