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Suppression of high-fat-diet-induced obesity in mice by dietary folic acid supplementation is linked to changes in gut microbiota

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(2011) proposed a sandwich layer method in which colonies from soil samples are first cultured on medium A, then the whole agar of medium A is lifted from the plate and transferred to the surface of medium B (containing the methylene blue indicator), thus forming a medium A-colonies- medium B sandwich. After incubation for 2 h at 40C, strains forming haloes are then picked and purified

7 Annual Report on the Aging Society, 2020 8 Grants-in-Aid for Scientific Research, MHLW Special Research Project