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However, ACC1 is located upstream of FA synthesis and regulates the conversion of acetyl-CoA to malonyl-CoA, whereas SCD1 is located in the FA desaturation link and directly affects membrane lipid composition and oxidative sensitivity

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After centrifugation at 20,400 g for 10 min at 4 C, the supernatant was transferred to a new tube, and Flag-Syt I or II was immunoprecipitated with the anti-Flag antibody and Dynabeads protein G (Veritas, Mountain View, CA, USA

Wang, K., Dang, W., Yan, J., Chen, R., Liu, X., Yan, W., et al., Antimicrob