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The following formula was used to calculate percentage cytotoxicity [22]: Measurements of malondialdehyde and iron levels Intracellular malondialdehyde (MDA) content was measured using a fluorometric lipid peroxidation assay kit (Abcam, 118970) following the manufacturers instructions

Cost-effective alternatives to organoids and 3D cultures, as well as standardized protocols for their generation and use, are crucial for ensuring reproducibility of results and equitable research opportunities across institutions with varying resources

Affected Muscle Groups Muscle spasms often occur in larger muscle groups like those in the back, legs, or arms

These effects occur through mechanisms including enhanced angiogenesis, modulation of growth factor expression, and direct cytoprotection of gastric epithelial cells