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The phosphopeptides were eluted by adding 80 L of elution buffer directly (50% acetonitrile, 500 uL H 2 O, 7% Nh4OH ammonium hydroxide)
Metformin users, common among type 2 diabetes patients who then add semaglutide, face a double hit since metformin independently reduces B12 absorption by 10-30% over long-term use
