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Description
S.GlasbeyT.KrielF
Broadly speaking, inflammatory M1 macrophages rely predominantly on glycolysis and glucose-driven pathways while reparative M2 macrophages predominantly utilize FAO and drive OXPHOS
In brief, cells from nearly confluent 15-cm plates treated with 200 M H 2 O 2 for 30 min were harvested and lysed in IP buffer (20 mM HEPES, 150 mM NaCl, 2 mM MgCl 2 , 0.5 mM CaCl 2 , 1% (v/v) Brij-35, and protease inhibitor cocktail)
