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Biochemistry 46:22752288 Hirst J, Carroll J, Fearnley IM, Shannon RJ, Walker JE (2003) The nuclear encoded subunits of complex I from bovine heart mitochondria

Potential limitations include scalability issues, safety concerns, and the need for additional infrastructure relative to conventional methods
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coli has distinct GshA and GshB proteins, while Listeria monocytogenes features a single bifunctional enzyme, GshF, which catalyzes both steps within one polypeptide