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Immunoblotting For protein extraction, the cells were washed twice with ice-cold 1 PBS and lysed in 50 mM Tris pH 7.5, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, supplemented with protease and phosphatase inhibitors followed by sonication
It is but justice to Colonel Butler to say, that he was far more humane than his son Walter, and that his personal deeds at Wyoming were not so heinous as the common accounts have made them

TT and CS acknowledge support from the Academy of Finland (grants 327732 and 331837)

Abnormal degradation of the neuronal stress-protective transcription factor HSF1 in Huntingtons disease
