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Long-term expansion of functional mouse and human hepatocytes as 3D organoids

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Thereafter, the beads were washed three times with 1 ml NP-40 wash buffer (50 mM Tris/HCl, pH 7.5, 400 mM NaCl, 0.5 % (w/v) NaDOC, and 0.5 % (v/v) NP-40), including centrifugation steps for 5 min at 4 C and 620 g (2.500 rpm) in between
