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Isolation and in vitro propagation of tumorigenic breast cancer cells with stem/progenitor cell properties

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Required materials: BPC 157 lyophilized vial (typically 5 mg or 10 mg) Bacteriostatic water (BAC water, 0.9% benzyl alcohol) for injection U-100 insulin syringes (1 mL capacity, 2831 gauge) Alcohol swabs (70% isopropyl) Sterile nitrile gloves A clean, flat workspace (ideally wiped with 70% isopropyl alcohol) Sharps disposal container Workspace and vial preparation: Warming the lyophilized vial to room temperature for 1020 minutes before opening reduces condensation on the stopper