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Quantitative real-time PCRs were done with FastStart Universal SYBR Green Master Mix (Roche, Basel, Switzerland) and ABI 7300 thermal cycler (Applied Biosystems, Foster City, CA, USA), or with PowerUp SYBR Green Master Mix (Applied Biosystems) and QuantStudio 5 real-time qPCR thermal cycler (ThermoFisher Scientific)

10.1530/endoabs.109.EP40 52 MiyamotoT.HiramotoS.KantoA.TsubotaM.FujitaniM.FukuyamaH.et al (2021)

If youre considering adding coffee enemas into a broader detox or gut-healing plan, working with a qualified functional medicine provider ensures youre doing it safely and effectively.These individuals should not perform coffee enemas: Those with inflammatory bowel disease (Crohns, UC) Severe hemorrhoids Kidney disease Heart rhythm disorders Pregnant individuals Those sensitive to caffeine Consult a licensed medical provider before starting any detox or gastrointestinal protocol

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