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Boardman HMP et al

MOTS-c levels decline with age and with metabolic dysfunction

The fluids were centrifuged at 8000 rpm for 20 min at 4 C to remove cellular debris and 25 l of clear supernatant was treated with 4X Laemmli buffer (to a final concentration of 1X)

3-Hydroxyacyl-coenzyme A dehydrogenase deficiency and hyperinsulinemic hypoglycemia: characterization of a novel mutation and severe dietary protein sensitivity