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Every time a cell divides, its telomerestiny DNA sequences at the ends of chromosomesbecome slightly shorter

On the experimental day, cells were washed twice and placed into KRB buffer (132.2 mM NaCl, 3.6 mM KCl, 5 mM NaHCO 3 , 0.5 mM NaH 2 PO 4 , 0.5 mM MgCl 2 , 1.5 mM CaCl 2 and 0.001 g/mL BSA, pH 7.4) supplemented with 25 mM glucose (1 h, 37 C, 5% CO 2 )

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