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Next, 50 L of a 5% phenylisothiocyanate (PITC) solution was added to derivatize the amino acids and biogenic amines

Enzyme-linked immunosorbent assay (ELISA) Spinal cord tissue and muscle tissue were weighted and quickly homogenized in ice cold PBS according to manufactures protocols followed by centrifugation at 4500 g for 5 min at 4 C

E1 is a thiamin pyrophosphate (TPP)-dependent -ketoacid dehydrogenase, R -lipoic acid functions as a prosthetic group essential for E2 transacetylase activity, and E3 is a flavin adenine dinucleotide (FAD)-dependent dihydrolipoamide dehydrogenase ( Figure 4 )

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