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However, this resistance can be mitigated by oxidized phosphatidylcholine (OxPC) produced during ferroptosis, which inhibits iNOS production by preventing NF-B binding to the iNOS promoter (Friedl et al

While traditional methods like protein precipitation, liquidliquid extraction, and solid-phase extraction (SPE) remain common, newer techniques (such as microextraction, microdialysis, and automated sample preparation) are gaining popularity due to their minimal sample loss, reduced matrix interference, and improved efficiency [40, 41]

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