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Professors Marshall-Gradisnik and Staines from Australia have published a potential mechanism of action for LDN in ME/CFS involving a calcium (Ca 2+ ) ion channel called TRPM3 and opioid receptor interaction in natural killer cells
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Immunofluorescent staining of cells After treatment, cells was fixed for 15 min using fresh, methanol-free 4% formaldehyde, and then, rinsed thrice with PBS for 5 min each
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