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To examine the extent to which the phenotypes observed were specifically caused by lower GSH levels, we rescued GSH depletion in liver-specific Gclc KO mice with GSH mono ethyl ester (GSH-ee)

Pathological immobilisation of iron by protein aggregates such as amyloid and tau may sequester iron in a non-bioavailable form, or impaired autophagy may prevent efficient breakdown of ferritin to mobilise iron, thereby impairing mitochondrial ATP production and subsequently glutathione synthesis, an essential component of the ferroptosis defence system [29]

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