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Tissue sections that were 4 m thick were sliced from the paraffin blocks using a microtome (Leica RM2245, Leica Microsystems, Wetzlar, Germany) and stained with hematoxylin and eosin for histopathological evaluations

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If you have feelings of hopelessness, guilt, numbness, grief, and loneliness, theres a chance youre suffering from depression