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Bronchoalveolar lavage sample for analysis of immune cells, inflammatory cytokines, and neutrophilic extracellular traps The BAL supernatant was examined for various inflammation markers (e.g., tumor necrosis factor [TNF]-, interleukin [IL]-1, IL-6, IL-8, and IL-18) by using a ProcartaPlex Multiplex Immunoassays Kit (Thermo Fisher Scientific) to perform quantitative, multiplexed protein measurements and using Luminex magnetic bead technology per manufacturer recommendations [30]

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