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Total RNA was harvested from monolayer and pellet cultures at different time points using Qiazol (Qiagen, Germantown, MD, United States) and purified with RNeasy Plus Universal Mini Kit (Qiagen, Cat
Additional primary antibodies used were anti--synuclein (AS) (1:1000, Chemicon or 1:50, Abcam) and anti-tyrosine hydroxylase (TH, 1:8000, Sigma)
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