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Antioxidant protective mechanisms against reactive oxygen species (ROS) generated by mitochondrial P450 systems in steroidogenic cells
2.6 Analysis of cytokines in tears and HCEpiC cell cultures To determine cytokine levels from recovered tears and supernatant from treated HCEpiC cells, the LEGENDplex Human Inflammation Panel 1 (13-plex) flow cytometry-based assay kit was used (BioLegend, San Diego, CA)
It yields an overall score and sub-section scores (physical mobility, energy, pain, emotional reactions, sleep, and social isolation)
doi: 10.3390/cancers10010026
