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The hMOs featured a central stem cell niche, with asymmetrically distributed dopaminergic neurons, and expressed midbrain floorplate markers FOXA2 and EN1
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It frequently exhibits neurofibromin ( NF1 ) mutations and deletions at 17q11.2 (further affecting NF1 expression), often co-occurring with phosphatase and tensin homolog ( PTEN ) mutations and phosphatidylinositol 3-kinase (PI3K)/AKT pathway enrichment [15,80]
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